Ghrp-6 & CJC 1295

[thomas]

These peptides will be next up for testing for me...any one tried them ??

Am still reading up just now I will post up a summary of what i find when I get a chance

Most of the things I am looking into are to help with recovery for my shoulder.

[SW1]

youre having a good bash with the peptides lately arent ya thomas
think i`ll just stick with my steds and growth
some of the new stuff scares me shitless tbh..lol

[thomas]

TBH growth would have been my first choice...... but I decided to explore other avenues as well. I will post up what I found in the next couple of days just need to get it straight in my head first...quite interesting with good reports all round but its all new and shiney so no long term feed back ie 5 years plus.

It looks like I may have to take up to six weeks off work after surgery on SSP... ie pennies so recovery may need to be economical as well as fun.

[thomas]

After a good bit of reading there would seem to be positive all round for the ghrp-6 (basically poor man's GH) but conflicting reports for cjc mainly as its unlikely to ever actually get real cjc and mixing both together will end up with your body all over the place when you come off. Unfortunitly it would seem to be like hens teeth to get ahold of up here so cant really try in out.

One of the better articles I came across......

What is growth hormone?

Synthetic Growth Hormone is an artificially created hormone "identical" to the major naturally produced (endogenous) isoform. It is often referred to by its molecular mass which is 22kDa (kilodaltons) and is made up of a sequence of 191 amino acids (primary structure) with a very specific folding pattern that comprise a three-dimensional structure (tertiary structure). This tertiary structure is subject to potential shape change through a process known as thermal denaturation. While many labs are capable of generating growth hormone (GH) with the proper primary structure not all will be capable of creating a tertiary structure identical to the major naturally occurring growth hormone. The tertiary structure can determine the strength with which the growth hormone molecule binds to a receptor which will in turn affect the "strength" of the intracellular signaling which mediates the events leading to protein transcription, metabolism, IGF-1 creation, etc. It is this inconsistency that accounts in part for the differences in effectiveness of various non-pharmaceutically produced synthetic growth hormone.

Naturally produced Growth Hormone is produced in the anterior pituitary and to a far lesser extent in peripheral tissue. It is made up of a blend of isoforms the majority of which is the 22kDa (191 amino acid) variety with which most are familiar. In addition an isoform that is missing the 15 amino acids that interact with the prolactin receptor is also produced. This form is known as 20kDa and although it binds differently to the growth hormone receptor it has been shown to be equally potent to 22kDa. It appears that 20kDa has lower diabetogenic activity then 22kDa. The pituitary releases a blend of these two isoforms with 20kDa averaging perhaps 10% of the total although this percentage increases post-exercise. Currently there is no synthetic produced for external administration for this isoform.

Growth hormone (GH) in the body is released in pulsatile fashion. It has been demonstrated that this pattern promotes growth. The pituitary is capable of rather quickly synthesizing very large amounts of growth hormone which it stores large amounts in both a finished and unfinished form. Adults rarely experience GH pulses (i.e. releases of pituitary stores) that completely deplete these stores. As we age we do not lose the ability to create and store large amounts of growth hormone. Rather we experience a diminished capacity to "instruct" their release. The volume of GH that is released can not be properly equated to the exogenous administration of synthetic GH for the reason that a set of behavioral characteristics accompany natural GH that differ from those of synthetic GH. Among those characteristics are concentrated pulsatile release which upon binding in mass to growth hormone receptors on the surface of cells initiate signaling cascades which mediate growth events by translocating signaling proteins to the nucleus of the cell where protein transcription and metabolic events occur.

These very important signaling pathways desensitize to Growth Hormone's initiating effects and need to experience an absence of Growth Hormone in order to reset and be ready to act again. The presence of GH released in pulsatile fashion is graphed as a wave with the low or no growth hormone period graphed as a trough. Therefore attempting to find a natural GH to synthetic GH equivalency is not very productive because in the end what is probably import is:
- the quantity & quality of intracellular signaling events; and
- the degree to which GH stimulates autocrine/paracrine (locally produced/locally used) muscle IGF-1 & post-exercise its splice variant MGF.
Synthetic GH versus Natural GH in IUs

An attempt has been made on my part and can be found at:
#8 - Growth Hormone Administration vs. CJC-1295/GHRP-6 + GHRH (part I of II)

#9 - Growth Hormone Administration vs. CJC-1295/GHRP-6 + GHRH (part II of II)
Rather than demonstrate absolute values this comparison articles should serve to demonstrate that the body can produce pharmacological levels of growth hormone.

Brief overview of natural GH release

The initiation of growth hormone release in the pituitary is dependent on a trilogy of hormones:
Somatostatin which is the inhibitory hormone and responsible in large part for the creation of pulsation;

Growth Hormone Releasing Hormone (GHRH) which is the stimulatory hormone responsible for initiating GH release; and

Ghrelin which is a modulating hormone and in essence optimizes the balance between the "on" hormone & the "off" hormone. Before Ghrelin was discovered the synthetic growth hormone releasing peptides (GHRPs) were created and are superior to Ghrelin in that they do not share Ghrelin's lipogenic behavior. These GHRPs are GHRP-6, GHRP-2, Hexarelin and later Ipamorelin all of which behave in similar fashion.
In the aging adult these Ghrelin-mimetics or the GHRPs restore a more youthful ability to release GH from the pituitary as they turn down somatostatin's negative influence which becomes stronger as we age and turn up growth hormone releasing hormone's influence which becomes weaker as we age.

The exogenous administration of Growth Hormone Releasing Hormone (GHRH) creates a pulse of GH release which will be small if administered during a natural GH trough and higher if administered during a rising natural GH wave.

Growth Hormone Releasing Peptides (GHRP-6, GHRP-2, Hexarelin) are capable of creating a larger pulse of GH on their own then GHRH and they do this with much more consistency and predictability without regard to whether a natural wave or trough of GH is currently taking place.

Synergy of GHRH + GHRP

It is well documented and established that the concurrent administration of Growth Hormone Releasing Hormone (GHRH) and a Growth Hormone Releasing Peptide (GHRP-6, GHRP-2 or Hexarelin) results in synergistic release of GH from pituitary stores. In other words if GHRH contributes a GH amount quantified as the number 2 and GHRPs contributed a GH amount quantified as the number 4 the total GH release is not additive (i.e. 2 + 4 = 6). Rather the whole is greater than the sum of the parts such that 2 + 4 = 10.

While the GHRPs (GHRP-6, GHRP-2 and Hexarelin) come in only one half-life form and are capable of generating a GH pulse that lasts a couple of hours re-administration of a GHRP is required to effect additional pulses.

Growth Hormone Releasing Hormone (GHRH) however is currently available in several forms which vary only by their half-lives. Naturally occurring GHRH is either a 40 or 44 amino acid peptide with the bioactive portion residing in the first 29 amino acids. This shortened peptide identical in behavior and half-life to that of GHRH is called Growth Hormone Releasing Factor and is abbreviated as GRF(1-29).

GRF(1-29) is produced and sold as a drug called Sermorelin. It has a short-half life measured in minutes. If you prefer analogies think of this as a Testosterone Suspension (i.e. unestered).

To increase the stability and half-life of GRF(1-29) four amino acid changes where made to its structure. These changes increase the half-life beyond 30 minutes which is more than sufficient to exert a sustained effect which will maximize a GH pulse. This form is often called tetrasubstituted GRF(1-29) (or modified) and unfortunately & confusingly mislabeled as CJC-1295. If you prefer analogies think of this as a Testosterone Propionate (i.e. short-estered).

Note that some may also refer to this as CJC-1295 without the DAC (Drug Affinity Complex).

Frequent dosing of either the aforementioned modified GRF(1-29) or regular GRF(1-29) is required and as previously indicated works synergistically with a GHRP.

In an attempt to create a more convenient long-lasting GHRH, a compound known as CJC-1295 was created. This compound is identical to the aforementioned modified GRF(1-29) with the addition of the amino acid Lysine which links to a non-peptide molecule known as a "Drug Affinity Complex (DAC)". This complex allows GRF(1-29) to bind to albumin post-injection in plasma and extends its half-life to that of days. If you prefer analogies think of this as a Testosterone Cypionate (i.e. long-estered)

CJC-1295 is difficult to produce and expensive to make. As a result it could be cost-prohibitive to use extensively. Modified GRF(1-29) while less convenient is much less expensive to make and because it is a pure peptide the synthesis process is straightforward. It should sell at a fraction of the cost of CJC-1295.

What follows on this first page of the thread is:

- A Basic Peptide Primer (which introduces the concept & structure of peptides)

- A Brief Summary of Dosing and Administration (for someone that wants to know the "how to use" straight away)

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The Amino Acid Structures of Peptides discussed in this thread

Growth Hormone Releasing peptides (GHRPs) (GH pulse initiators):
- GHRP-6 (His-DTrp-Ala-Trp-DPhe-Lys-NH2)

- GHRP-2 (DAla-D-2-Nal-Ala-Trp-DPhe-Lys-NH2)

- Hexarelin (His-D-2-methyl-Trp-Ala-Trp-DPhe-Lys-NH2)

- Ipamorelin (Aib-His-D-2-Nal-DPhe-Lys-NH2) - Ref-1
NOTES:
Aib = Aminoisobutyryc acid
D-2-Nal = "D" form of 2’-naphthylalanine
Growth Hormone Releasing Hormone (GHRH) (amplifies the GHRP initiated pulse):
- Growth Hormone Releasing Hormone (GHRH) aka GRF(1-44) (Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-NH2) = half-life "less then 10 minutes", perhaps as low as 5 minutes. - Ref-2


- GRF(1-29) aka Sermorelin (Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2) - the biologically active portion of the 44 amino acid GHRH = half-life "less then 10 minutes", perhaps as low as 5 minutes. - Ref-3
- Longer-lasting analogs of GRF(1-29):
-- replace the 2nd amino acid Alanine w/ D-Alanine only to modify GRF(1-29), D-Ala2 GRF(1-29) (Tyr-DAla-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2) = half-life "closer to 10 minutes" - Ref-4


-- replace the 2nd, 8th, 15th & 27th amino acids & get modified GRF(1-29) or CJC-1295 w/o the DAC (i.e. the part that will bind to albumin & make the half-life days) (Tyr-DAla-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2) = Half-life at least 30 minutes or so - Ref-5

-- CJC-1295 (Tyr-DAla-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys-(Maleimidopropionyl)-NH2) = Half-life measured in days, - Ref-6
NOTES:
Lys = linker to the Drug Affinity Complex (aka (Maleimidopropionyl))
"Since GH is released in a pulsatile manner and a higher level of GH is observed between 15 and 30 min after subcutaneous administration of GH-RH analogues, hydrolysis by trypsin-like enzymes could not affect the result of stimulation." - Potent Trypsin-resistant hGH-RH Analogues, JAN IZDEBSKI, J. Peptide Sci. 10: 524–529 (2004)
The analog in the above quoted study resisted degradation for 30 minutes. The quote implies that if your analog can last 30 minutes it has tapped out the potential for a single pulse.

Since another pulse won't be generated for about 2.5 - 3 hours analogs that last more than 30 minutes up to 3 hours are not any more beneficial.

You would need an analog that kept growth hormone releasing hormone around beyond 3 hours to have it trigger a second pulse.

Otherwise dosing the 30 minute analog every 3 hours will maximize GH output OR you could just use an analog such as CJC-1295 which lasts for many days and will trigger several GH pulses a day for several days on a single dose.
References:

Ref-1 - "lack of effect on ACTH and cortisol plasma levels" - Ipamorelin, the first selective growth hormone secretagogue , K Raun, European Journal of Endocrinology, 1996 Vol 139, Issue 5, 552-561

Ref-2 - Rapid enzymatic degradation of growth hormone-releasing hormone by plasma in vitro and in vivo to a biologically inactive product cleaved at the NH2 terminus, Frohman LA, J Clin Invest. 1986 78:906–913 and Incorporation of D-Ala2 in Growth Hormone-Releasing Hormone-( l-29)-NH2 Increases the Half-Life and Decreases Metabolic Clearance in Normal Men, STEVEN SOULE, Journal of Clinical Endocrinology and Metabolism 1994 Vol. 79, No. 4

Ref-3 - Rapid enzymatic degradation of growth hormone-releasing hormone by plasma in vitro and in vivo to a biologically inactive product cleaved at the NH2 terminus, Frohman LA, J Clin Invest. 1986 78:906–913 and Incorporation of D-Ala2 in Growth Hormone-Releasing Hormone-( l-29)-NH2 Increases the Half-Life and Decreases Metabolic Clearance in Normal Men, STEVEN SOULE, Journal of Clinical Endocrinology and Metabolism 1994 Vol. 79, No. 4

Ref-4 - Incorporation of D-Ala2 in Growth Hormone-Releasing Hormone-( l-29)-NH2 Increases the Half-Life and Decreases Metabolic Clearance in Normal Men, STEVEN SOULE, Journal of Clinical Endocrinology and Metabolism 1994 Vol. 79, No. 4

Ref-5 - See: Posts within this thread

Ref-6 - See: Posts within this thread
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

A Brief Summary of Dosing and Administration

Dosing GHRPs

The saturation dose in most studies on the GHRPs (GHRP-6, GHRP-2, Ipamorelin & Hexarelin) is defined as either 100mcg or 1mcg/kg.

What that means is that 100mcg will saturate the receptors fully, but if you add another 100mcg to that dose only 50% of that portion will be effective. If you add an additional 100mcg to that dose only about 25% will be effective. Perhaps a final 100mcg might add a little something to GH release but that is it.

So 100mcg is the saturation dose and you could add more up to 300 to 400mcg and get a little more effect.

A 500mcg dose will not be more effective then a 400mcg, perhaps not even more effective then 300mcg.

The additional problems are desensitization & cortisol/prolactin side-effects.
Ipamorelin is about as efficacious as GHRP-6 in causing GH release but even at higher dose (above 100mcg) it does not create prolactin or cortisol.

GHRP-6 at the saturation dose 100mcg does not really increase prolactin & cortisol but may do so slightly at higher doses. This rise is still within the normal range.

GHRP-2 is a little more efficacious then GHRP-6 at causing GH release but at the saturation dose or higher may produce a slight to moderate increase in prolactin & cortisol. This rise is still within the normal range although doses of 200 - 400mcg might make it the high end of the normal range.

Hexarelin is the most efficacious of all of the GHRPs at causing an increase in GH release. However it has the highest potential to also increase cortisol & prolactin. This rise will occur even at the 100mcg saturation dose. This rise will reach the higher levels of what is defined as normal.
Desensitization
GHRP-6 can be used at saturation dose (100mcg) three or four times a day without risk of desensitization.

GHRP-2 probably at saturation dose several times a day will not result in desensitization.

Hexarelin has been shown to bring about desensitization but in a long-term study the pituitary recovered its sensitivity so that there was not long-term loss of sensitivity at saturation dose. However dosing Hexarelin even at 100mcg three times a day will likely lead to some down regulation within 14 days.
If desensitization were to ever occur for any of these GHRPs simply stopping use for several days will remedy this effect.

Chronic use of GHRP-6 at 100mcg dosed several times a day every day will not cause pituitary problems, nor significant prolactin or cortisol problems, nor desensitize.

GHRH

Now Sermorelin, GHRH (1-44) and GRF(1-29) all are basically GHRH and have a short half-life in plasma because of quick cleavage between the 2nd & 3rd amino acid. This is no worry naturally because this hormone is secreted from the hypothalamus and travels a short distance to the underlying anterior pituitary and is not really subject to enzymatic cleavage. The release from the hypothalamus and binding to somatotrophs (pituitary cells) happens quickly.

However when injected into the body it must circulate before finding its way to the pituitary and so within 3 minutes it is already being degraded.

That is why GHRH in the above forms must be dosed high to get an effect.

GHRH analogs

All GHRH analogs swap Alanine at the 2nd position for D-Alanine which makes the peptide resistant to quick cleavage at that position. This means analogs will be more effective when injected at smaller dosing.

The analog tetra or 4 substituted GRF(1-29) sometimes called CJC w/o the DAC or referred to by me as modified GRF(1-29) has other amino acid modifications. They are a glutamine (Gln or Q) at the 8-position, alanine (Ala or A) at the 15-position, and a leucine (Leu or L) at the 27-position.

The alanine at the 8th position enhances bioavailability but the other two amino substitutions are made to enhance the manufacturing process (i.e. create manufacturing stability).

For use in vivo, in humans, the GHRH analog known as CJC w/o the DAC or tetra (4) substituted GRF(1-29) or modified GRF(1-29) is a very effective peptide with a half-life probably 30+ minutes.

That is long enough to be completely effective.

The saturation dose is also defined as 100mcg.

Problem w/ Using any GHRH alone

The problem with using a GHRH even the stronger analogs is that they are only highly effective when somatostatin is low (the GH inhibiting hormone). So if you unluckily administer in a trough (or when a GH pulse is not naturally occurring) you will add very little GH release. If however you luckily administer during a rising wave or GH pulse (somatostatin will not be active at this point) you will add to GH release.

Solution is GHRP + GHRH analog

The solution is simple and highly effective. You administer a GHRH analog with a GHRP. The GHRP creates a pulse of GH. It does this through several mechanisms. One mechanism is the reduction of somatostatin release from the hypothalamus, another is a reduction of somatostatin influence at the pituitary, still another is increased release of GHRH from the brain and finally GHRPs act on the same pituitary cells (somatotrophs) as do GHRHs but use a different mechanism to increase cAMP formation which will further cause GH release from somatotroph stores.

GHRH also has a way of reciprocally reinforcing GHRPs action.

The result is a synergistic GH release.

The GH is not additive it is synergistic. By that I mean:
If GHRH by itself will cause a GH release valued at 2
and GHRP itself will cause a GH release valued at 5

Together the GH is not 7 (5+2) it turns out to say 16!
A solid protocol

A solid protocol would be to use a GHRP + a GHRH analog pre-bed (to support the nightime pulse) and once or twice throughout the day.

For anti-aging, deep restful restorative sleep, the once at night dosing is all you need. For an adult aged 40+ it is enough to restore GH to youthful levels.

However for bodybuilding or fatloss or injury repair multiple dosings can be effective.

The GHRH analog can be used at 100mcg and as high as you want without problems.

The GHRP-6 can always be used at 100mcg w/o problems but a dose of 200mcg will probably be fine as well.

Again desensitization is something to keep an eye on particularly with the highest doses of GHRP-2 and all doses of Hexarelin.
So 100 - 200mcg of GHRP-6 + 100 - 500mcg+ of a GHRH analog taken together will be effective.
This may be dosed several times a day to be highly effective.
A solid approach is a bit more conservative at 100mcg of GHRP-6 + 100mcg of a GHRH analog dosed either once, twice, three or four times a day.
When dosing multiple times a day at least 3 hours should separate the administrations.

The difference is once a day dosing pre-bed will give a youthful restorative amount of GH while multiple dosing and or higher levels will give higher GH & IGF-1 levels when coupled with diet & exercise will lead to muscle gain & fatloss.

Dose w/o food

Administration should ideally be done on either an empty stomach or with only protein in the stomach. Fats & carbs blunt GH release. So administer the peptides and wait about 20 minutes (no more then 30 but no less then 15 minutes) to eat. AT that point the GH pulse has about hit the peak and you can eat what you want.


And one on cjc.......bit of an advert though


CJC-1295
unleashing
The Beast
by increasing GH 10X

So you want to look like the Hulk or maybe you just want to be lean and muscular. So what's actually stopping you? Genetics... but what exactly is limiting your muscle growth, while at the same time limiting the burning of fat. Quite simply the answer is Myostatin. Myostatin is a direct negative regulator of the amount of skeletal muscle you have and how lean you are. Studies have proven this using mice with the myostatin knock out gene in which they don't have myostatin to limit muscle growth. These Mighty lean ripped mice are called double muscled and they look like mice on steroids, all without working out. The prized winning Belgian Blue and Piedmontese cattle have mutations of myostatin resulting in double muscling. Mutations in humans have been shown too have the same results.

Here are some myostatin links:

1) Myostatin Controlling Obesity and Insulin Resistance Obesity and insulin resistance

2) Muscle and MyostatinDouble muscling in cattle due to mutations in the myostatingene

3) Burn more Fat Suppression of body fat accumulation in myostatin-deficient mice

4) Myostatin in HumansChild with myostatin mutation



So what is CJC-1295 and what does it have to do with myostatin you ask... CJC-1295 is a long acting synthetic version of Growth Hormone Releasing Hormone (GHRH). 30mcg/kg to 60mcg/kg doses can increase gh levels up to 10X normal for 6 days or more with plasma IGF-1 concentrations by 3X for 9-11 days. The estimated 1/2 life is 5.8-8.1 days. After multiple doses the mean IGF-1 levels remain above base level for up to 28 days. Results show that 30mcg/kg CJC-1295 is equal to 5iu's of gh without the normal negative side effects from taking gh. In essence its much better to use CJC-1295 to make your body produce its own gh instead of taking a synthetic gh that also shuts down your normal production. So how does CJC-1295 and Myostatin relate. Growth hormone directly regulates myostatin. As growth hormone rises, myostatin lowers, which unleashes your potential for muscle growth. As myostatin lowers glucocorticoids effect on fat storage and muscle breakdown lowers. CJC-1295 dramatically raises gh levels which will dramatically lower myostatin which in turn will lower the effects of glucocorticoids fat storage and muscle breakdown. Now you know what has changed that has turned the Pro's into Freaks.

Studies to prove it:

1)Improve Cardiovascular Functions
http://jcem.endojournals.org/cgi/con...urcetype=HWCIT

2)Get Lean & Muscular
http://jcem.endojournals.org/cgi/con...urcetype=HWCIT


3) Burn FatGrowth Hormone–Releasing Hormone in HIV-Infected Men With Lipodystrophy
http://jama.ama-assn.org/cgi/content/abstract/292/2/210

4) Dosing and resultsResults of CJC-1295 in Healthy Adults
http://jcem.endojournals.org/cgi/con...urcetype=HWCIT

5) Anabolic GH's direct anabolic effect from lowering Myostatin
http://jcem.endojournals.org/cgi/con...urcetype=HWCIT

6) Burn Fat-Retain MuscleLower Myostatin and prevent the catabolic effects of glucocorticoids
http://endo.endojournals.org/cgi/con...urcetype=HWCIT

7) Prevent ObesityGlucocorticoids and Obesity
http://endo.endojournals.org/cgi/con...urcetype=HWCIT


Raising Testosterone levels lowers Growth Hormone Binding Protein. This protein binds up excess gh. Testosterone lowering GHBP if you read the full text you will figure out that it's the spike in Test levels above normal from the shots that lowers GHBP proving that supraphysiological test levels raise gh and lowers GHBP

As you can see androgens can directly and indirectly raise growth hormone levels resulting in lowering Myostatin which you already learned increases muscle mass. On a side note leptin has a huge role in fat storage and as gh raises, leptin lowers. I won't delve into discussing leptin cause it can be complicated so do a search to learn more...


CJC-1295
Anti-Aging
Lets go beyond muscle building and fat loss and discuss the well documented anti-aging benefits of growth hormone. Since this info is so plenty full online I'll only outline the known benefits and let you fulfill you quest for Anti-Aging by doing your own research. The rate of GH secretion from the pituitary gland is at its peak around puberty, declining progressively as we age beyond that point. Around the late 30s and throughout the 40s, GH levels continue to dip considerably, causing the telltale signs of aging to appear. Fat tissue begins to collect in areas where it may have not before, the skin begins to lose thickness, bone mass becomes depleted, energy levels drop, and mental clarity may become clouded.
For the purposes of anti-aging therapy, GH treatment functions to re-stimulate cell production to youthful levels restoring form and function to the entire body, from the inside out. Those who have undergone successful anti-aging treatment with GH or a combination of GH and other diminishing hormones such as testosterone have been reported to experience improvement in the skin’s appearance, renewed energy, mental acuity, invigorated health, enhanced sexual function, an overall sense of well being and a more youthful lean muscled look. Hundreds of studies have been done on the controlled administration of true HGH in adults. It has been shown to raise energy levels, enhance lean muscle development, decrease body fat, strengthen the heart, improve cholesterol and triglyceride levels and HDL/LDL ratios, fortify bones, smooth out skin wrinkles, sharpen memory, improve sexual function, regenerate damaged tissue, strengthen the immune system as well as improve sleep.
Somatotropin (HGH) from the pituitary gland works by stimulating protein production and synthesis in all tissues of the body. This results in an increase in organ healing and regeneration, which is associated with increase resistance to illness and injury.
In the skin, documentation regarding somatotropin (HGH) supplementation has shown increased collagen synthesis, which makes the skin thicker and smoother. In the bone, these studies showed increased bone density. In the internal organs and sex organs, research showed increased size and markedly improved function.
The immune system, research also showed increased immune system regulation and activity, resulting in fewer infectious illnesses. In adults, HGH is necessary for proper repair and regeneration of injured tissues.
One of the widely reported results of somatotropin (HGH) supplementation is the effect it has on the body's sexual centers. Research shows heightened desire and function in both men and women.
Beginning around the age of 20 years old, our natural production of somatotropin (HGH) begins to decline at the average rate of 14% per decade.
By the age of 40, somatotropin (HGH) production has decreased 30% over our youthful production level, and by the age of 60, by 80% on average. The decline in somatotropin (HGH) correlates with many of the effects of aging including loss of lean body mass (muscle, bone, skin and internal organs), increase in total body fat, thinning and wrinkling of skin, decreased immune function and decreased sexual desire and function.
As a result of the decline in somatotropin (HGH), and because it is the master hormone, other hormones also decline with age, such as Testosterone, Estrogen, Progesterone and DHEA, as well as many other important mood-elevating hormones. Examples of other hormones that also decrease are thyroid and thymus hormones, which can cause menopause in women and andropause in men.


Growth Hormone can:
Restore muscle loss
Increase skin thickness
Reduce total body fat
Improve bone density


People have seen specific improvements from using HGH in:


Skin tightness and moisture
Mental speed and clarity
Enhanced sexual function
Elevated mood and energy levels
Improved aerobic capacity
Significantly greater immune function
Increased wound healing capacity

So, gh therapy sounds great for anti-aging so what's the catch... The catch is keeping your levels in a physicological level and to not exceed youthful levels. As with any hormone, raising levels above youthful normal levels can cause unwanted side effects which are easily noticed like joint problems, the development of carpel tunnel and excess water weight gain. Its simple, just lower your dose if you notice a problem. There is a huge difference between keeping levels well above normal for years for anti-aging and doing a cycle with gh/cjc-1295 that exceed normal levels. Short term higher levels have much less potitential to cause long term problems... Ok, so you are interested in anti-aging with cjc-1295, what dose should you use. When taking into consideration the studies on it's outcome of gh production, as little as 1mg weekly is needed and no more then 2mg a week. This makes CJC-1295 extremely cost effective. Vials could be shared with fellow researchers if only 1mg is used weekly. The lab states after reconsituting cjc-1295 it can be refrozen to be used later but is best used without refreezing, so there may be a slight potiency loss by doing this.

Dosing for Muscle Growth
In the studies 30mcg/kg or 60mcg/kg were used resulting in 2-10 fold in gh production. A 200lb man weighs aprox 90kg... thats 200 divided by 2.2 = 90 so thats 30mcg X 90=2700mcg=2.7mg or 60mcg X 90=5400mcg=5.4mg weekly these doses showed safety... I believe a minimum 2mg a week should be used and no more then 6mg... You decided but I say start low and work your way up to the dose you are comfortable with and remember to keep this short term to reach a specific goal and then go on an anti-aging dose between cycles which would also preserve the muscle growth you build during the cycle.

[Steddy Eddie]

another good read thomas.

ive always been under the impression growth was not a real "mass building" drug.. but the article seems to make out its like a key unlocking the gates to a myostatinless freakshow! So I dont know what to think any more..

[thomas]

another good read thomas.

ive always been under the impression growth was not a real "mass building" drug.. but the article seems to make out its like a key unlocking the gates to a myostatinless freakshow! So I dont know what to think any more..

Tbh mate I still don't think its a great mass builder...you wont beat AAS for mass but it does offer some very good results in different areas, Tendons and a feeling of well being for me.

I been using 2iu a day before bed for 3 months ( will continue for 3 more ) now....nothing startling result wise but I am noticing small differences. I am using it for recovery purpose's for my shoulder nothing else and so far I think it is definitely helping. Is it worth the cash?? I don't know to be honest . I still want to try GHRP-6 or GHRP-2 but was next to imposable to get a hold of up here. Should be roughly half the price of GH so result dependant that may be worth the cash.